Speaker
Description
With the development of the aerospace industry, the duration of astronauts' stay in space has significantly increased, and space radiation has become one of the major threats to their health. Vascular endothelial cells are relatively sensitive to ionizing radiation, and their dysfunction is closely associated with the occurrence of cardiovascular diseases. Currently, the damage mechanism of proton radiation to organisms, especially to the cardiovascular system, remains unclear. In this study, human umbilical vein endothelial cells (HUVEC) were used as the research object. Relying on the 100 MeV proton cyclotron at the China Institute of Atomic Energy, HUVEC were irradiated with protons at four doses of 0.25, 0.5, 0.75, and 1 Gy. The effects on the results of cell transcriptional sequencing in each dose - group were detected and analyzed. Differential gene expression analysis showed that the number of differentially expressed genes increases with the rise in dose. GO and KEGG enrichment analysis results indicate that at the 0.25 Gy dose, cells exhibit a pro-inflammatory and anti-inflammatory balance. At the 0.5 Gy dose, inflammatory responses remain enriched while the necrotic apoptosis pathway is activated. At the 0.75 Gy dose, immune-inflammatory dysregulation occurs. At the 1 Gy dose, immune-inflammatory dysregulation intensifies alongside DNA damage enrichment.
Keywords: proton radiation, HUVEC, inflammatory damage, transcription sequencing analysis
