Speaker
Description
A new hard x-ray fluorescence nanoprobe instrument called Bionanoprobe-II (BNP-II) has been designed and will be constructed at 2-ID-D of the upgraded Advanced Photon Source. BNP-II will take advantage of the orders-of-magnitude increase in brightness and coherent flux with advanced sample scanning, metrology, cryogenics, and controls. These advancements will enable high-throughput XRF imaging under cryogenic conditions with 10 nm spatial resolution, 2D survey of mm-sized samples, and fast tomography for 3D visualization. BNP-II also introduces a novel robotic sample transfer system that interconnects a cryogenic plasma focused ion beam (cryo-PFIB) milling station alongside the x-ray nanoprobe. The interconnected instruments enable an iterative workflow between x-ray measurements and cryo-PFIB milling and maintains the integrity of vitrified samples by remaining below 110K even during transfer. Regions of interest can be identified by fast large-area scans, after which the sample geometry can be optimized for nanoscale x-ray imaging and tomography. This work details the engineering advancements required to examine highly complex, multidimensional systems with BNP-II.
Funding Agency
This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science user facility under Contract No. DE-AC02-06CH1135
