The design of SIBIPIRUNA, a cryogenic soft X-ray tomography beamline with BSL2, BSL3 and BSL4 compatibility for Sirius and Orion at CNPEM

TUO05
16 Sept 2025, 14:20
20m
The Loop

The Loop

Lund, Sweden
Contributed Oral Presentation Beamlines and Instruments Beamlines Session 1

Speaker

Renan Ramalho Geraldes (Brazilian Synchrotron Light Laboratory)

Description

Recent outbreaks of emerging infectious diseases have highlighted the need for enhanced biosafety measures and research capabilities. Addressing this, the Brazilian Center for Research in Energy and Materials(CNPEM) is spearheading the development of Orion, Latin America’s first facility to host a Biosafety Level 4(BSL4) laboratory$*$. More ambitiously, Orion will pioneer a groundbreaking global achievement: the integration between BSL4 areas and synchrotron beamlines. A connection between Orion and the 4th-generation storage ring Sirius/LNLS$**$ will enable unprecedented X-ray bioimaging opportunities in soft,tender and hard X-rays, with a program covering cells, tissues up to entire organisms. At the lower energy range, the SIBIPIRUNA beamline will allow for 3D imaging of infected single cells using cryogenic soft X-ray tomography. With a resolution target of 30nm, rapid full tomography time around 5 to 10 minutes, and whole unstained samples, unmatching detailed studies of viral infection mechanisms will be unlocked. This work describes the design of the beamline and its end-stations, highlighting their compatibility and compliance with biocontainment and decontamination needs.

Funding Agency

Brazilian Ministry of Science, Technology and Innovation (MCTI)

Footnotes

$*$ M. Rodrigues, 2024, Nature 632.8027
$**$ L. Liu et al., 2023, Proc. 14th Int. Particle Acc. Conf., pages 2586–2589

Author

Renan Ramalho Geraldes (Brazilian Synchrotron Light Laboratory)

Co-authors

Artur Clarindo Pinto (Brazilian Synchrotron Light Laboratory) Bernd Christian Meyer (Brazilian Synchrotron Light Laboratory) Erik Olivi Pereira (Brazilian Synchrotron Light Laboratory) Gabriel Antunes Souza (Brazilian Synchrotron Light Laboratory) Gabriel Gonçalves Basilio (Brazilian Synchrotron Light Laboratory) Gustavo Lorencini M. P. Rodrigues (Brazilian Synchrotron Light Laboratory) Harry Westfahl Jr (Brazilian Synchrotron Light Laboratory) Lucas Sanfelici (Brazilian Synchrotron Light Laboratory) Mairon Oliveira Lima (Brazilian Synchrotron Light Laboratory) Marco Orlando Eneas Leal (Brazilian Synchrotron Light Laboratory) Mateus Borba Cardoso (Brazilian Synchrotron Light Laboratory) Michel Bernardino Machado (Brazilian Synchrotron Light Laboratory) Pedro Pereira da Rocha Proença (Brazilian Synchrotron Light Laboratory) Renata Santos Rabelo (Brazilian Synchrotron Light Laboratory)

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